Antigen persists in discrete cell subsets within the lymph node
Ag-low and -high cells were identified by separately clustering each d14 LEC subset for each sample into two groups based on Ag-score. The cutoffs identified for the d14 dataset were then used to identify Ag-high cells for the other timepoints. With this approach there is a different cutoff for each LEC subset, but the cutoff is consistent across timepoints.